BacMagic™-2 DNA Kit, MilliporeSigma

Supplier: EMD MILLIPORE (BIOSCIENCES)
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72157-3 70967-6 70967-3 70967-5
80018-132EA 938.97 USD
80018-132 80017-194 80511-356 80511-360
BacMagic™-2 DNA Kit, MilliporeSigma
Protein Expression Protein Expression Systems

The BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells


BacMagic-2 DNA is derived from an AcNPV genome,
contains an ORF 1629 deletion which prevents nonrecombinant virus from replicating in insect cells, and contains a deletion of the nonessential chitinase (chiA) gene, to improve production of secreted and membrane-targeted proteins. BacMagic-2 DNA have an additional deletion of the v-cath gene, a cathespsin-like cysteine protease, resulting in significantly improved quality and yield for most target proteins.


The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.


Ordering information: The BacMagic-2 DNA Kit contains 0.5 µg of the BacMagic-2 DNA, 50 µL of the Insect GeneJuice® Transfection Reagent, and 2 µg of a positive control plasmid to monitor co-transfection efficiency.

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