BacMagic™-2 DNA Kit, MilliporeSigma
Supplier: EMD MILLIPORE (BIOSCIENCES)Total Ratings: 0
Avg. Ratings: 0.0 out of 5
The BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells
BacMagic-2 DNA is derived from an AcNPV genome,
contains an ORF 1629 deletion which prevents nonrecombinant virus from replicating in insect cells, and contains a deletion of the nonessential chitinase (chiA) gene, to improve production of secreted and membrane-targeted proteins. BacMagic-2 DNA have an additional deletion of the v-cath gene, a cathespsin-like cysteine protease, resulting in significantly improved quality and yield for most target proteins.
The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.
Ordering information: The BacMagic-2 DNA Kit contains 0.5 µg of the BacMagic-2 DNA, 50 µL of the Insect GeneJuice® Transfection Reagent, and 2 µg of a positive control plasmid to monitor co-transfection efficiency.
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