ONETAQ® Hot Start DNA Polymerase, New England Biolabs

Supplier: W.u.G. Dickgießer GmbH
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M0481L M0481S M0481X
101640-974EA 426 USD
101640-974 101640-976 101640-978
ONETAQ® Hot Start DNA Polymerase, New England Biolabs
Nucleic Acid Reagents End-point PCR Enzymes and Kits

OneTaq® Hot Start DNA Polymerase is an optimized blend of Taq and Deep VentR™ DNA polymerases combined with an aptamer-based inhibitor.


The 3´→ 5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase and the hot start formulation combines convenience with decreased interference from primer dimers and secondary products. The aptamer-based inhibitor binds reversibly, blocking polymerase activity at temperatures below 45°C, allowing reactions to be set up at room temperature. OneTaq Hot Start DNA Polymerase is activated during normal cycling conditions, eliminating the need for a separate high temperature incubation step to activate the enzyme. OneTaq Hot Start DNA Polymerase can therefore be substituted into typical or existing Taq-based protocols. OneTaq Hot Start is provided with Standard Reaction Buffer, GC Reaction Buffer and High GC Enhancer.

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