MitoCapture™ Mitochondrial Apoptosis Detection Kit, Enzo Life Sciences
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Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoCapture™ Apoptosis Detection Kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial membrane potential.
The kit utilizes MitoCapture™, a cationic dye that fluoresces differently in healthy and in apoptotic cells
The fluorescent signals can be easily detected by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using the FITC channel for green monomers (Ex(max): 488 nm; Em(max): 530 nm + 30 nm), and the PI channel for red aggregates (Ex(max): 488 nm; Em(max): 590 nm + 42 nm). An assay is completed within 20 - 30 min.
In healthy cells, MitoCapture™ accumulates and aggregates in the mitochondria, giving them a bright red fluorescence. In apoptotic cells, MitoCapture™ cannot aggregate in the mitochondria due to the altered mitochondrial membrane potential, and thus it remains in the cytoplasm in its monomer form, fluorescing green.
Delivery information: Kit contains MitoCapture™ ReagentIncubation Buffer.
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