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Product Image-TS43472-0010

Urea 99+% for biochemistry

Supplier: THERMO FISHER SCIENTIFIC CHEMICALS
Description: Urea 99+% for biochemistry

Product Image-TS32738-0010

Urea 99.5% for molecular biology DNase-, RNase-, protease-free

Supplier: THERMO FISHER SCIENTIFIC CHEMICALS
Description: Urea 99.5% for molecular biology DNase-, RNase-, protease-free

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Urea ≥99%, white crystalline powder

Supplier: MP Biomedicals
Description: Urea is a mild agent usually used in the solubilization and denaturation of proteins.

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Urea ≥99%, white powder, Ultrapure

Supplier: MP Biomedicals
Description: Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex.

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Urea 98+%

Supplier: Thermo Scientific Chemicals
Description: Used as a protein denaturant

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Urea ≥99%, white prills, Ultrapure

Supplier: MP Biomedicals
Description: Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-Urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.
Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove Urea first. Even though some enzymes will tolerate small amounts of Urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The Urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis.
Dissolve Urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature

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Urea, white prills, Reagent Grade

Supplier: MP Biomedicals
Description: Urea is a prinicipal protein metabolite end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle and the major product for the removal of free ammonia (NH4+) in vivo.

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Urea ≥99.0% (by total nitrogen basis) for biochemical research

Supplier: TCI America
Description: [for Biochemical Research]
CAS Number: 57-13-6
MDL Number: MFCD00008022
Molecular Formula: CH4N2O
Molecular Weight: 60.06
Purity/Analysis Method: >99.0% (N)
Form: Crystal
Color: White
Melting point (°C): 135

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Urea ≥99.3%, REacton®

Supplier: Thermo Scientific Chemicals
Description: MDL: MFCD00008022 Beilstein Registry No.: 635724 Fieser: 1,1262 2,455

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Product Image-TS19746-0050

Urea 98%, pearls, Extra Pure

Supplier: THERMO FISHER SCIENTIFIC CHEMICALS
Description: Urea 98%, pearls, Extra Pure

Product Image-TS42458-1000

Urea 99% ACS

Supplier: THERMO FISHER SCIENTIFIC CHEMICALS
Description: Urea 99% ACS

Product Image-TS14075-0010

Urea 99.5% for analysis

Supplier: THERMO FISHER SCIENTIFIC CHEMICALS
Description: Urea 99.5% for analysis

Product Image-95017-130

Urea

Catalog Number: (95017-130)
Supplier: GE Healthcare - Life Sciences
Description: 500 g

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Urea, BiotechGrade

Supplier: Spectrum Chemicals
Description: Urea, BiotechGrade is an odorless and colorless solid that is an important nitrogen-containing substance found in mammal urine. In concentrations up to 10 M, Urea is a powerful protein denaturant as it disrupts the noncovalent bonds in the proteins. This property can be exploited to increase the solubility of some proteins. Spectrum offers highly pure reagents suitable for biochemical research and analysis. The critical parameters involved are absence of inhibitors such as traces of heavy metals as well as biochemical function tests for enzymes, coenzymes and enzyme substrates.

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Urea

Catalog Number: (80058-810)
Supplier: MilliporeSigma
Description: (Carbimide; carbonyl diamide). White solid. PROTECT FROM MOISTURE. End product of the urea cycle. A topical keratolytic agent. Soluble in H2O. RTECS YR6250000, CAS 57-13-6, M.W. 60.1. WARNING! May be carcinogenic/teratogenic.

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Urea ≥99.5%, OmniPur®, Millipore®

Supplier: MilliporeSigma
Description: Ideal for protein unfolding/denaturation.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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