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Description: Urea 99.0-100.5%, crystallised ACS, VWR Chemicals BDH®
Catalog Number: BDH4602-500G
Supplier: VWR International

Description: For protein solubilization and denaturation. DNase-, RNase-, protease-, and cyanate-free.
Catalog Number: JT4111-5
Supplier: AVANTOR PERFORMANCE MATERIAL LLC

Description: Crystals.
Catalog Number: MK8648-06
Supplier: AVANTOR PERFORMANCE MATERIAL LLC

Description: Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the activity of Proteinase K digestions. Effectively denatures RNA. For use in nucleic electrophoresis applications.
Catalog Number: 97061-918
Supplier: VWR

Description: Suitable for bioprocessing. BSE/TSE free. Endotoxin tested. Custom packaging and testing available. Change management and notification available. Samples available.
Catalog Number: VWRB568-50KG
Supplier: Avantor Performance Materials

Description: GMP manufactured.
Catalog Number: JT4206-7
Supplier: AVANTOR PERFORMANCE MATERIAL LLC

Description: Urea, U.S.P.
Catalog Number: JT4205-60
Supplier: AVANTOR PERFORMANCE MATERIAL LLC

Description: Urea, U.S.P.
Catalog Number: JT4208-F8
Supplier: AVANTOR PERFORMANCE MATERIAL LLC

Description: Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the acti
Catalog Number: 97063-798
Supplier: VWR

Description: A convenient pre-mixed solution that is easily diluted for buffer preparation under denaturing conditions.Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the activity of Proteinase K digestions.
Catalog Number: 97063-804
Supplier: VWR

Description: Meets USP requirements. Meets BP/Ph. Eur. and JP chemical specifications. GMP manufactured product.
Catalog Number: JT4203-7
Supplier: AVANTOR PERFORMANCE MATERIAL LLC

Description: Crystals. GMP manufactured.
Catalog Number: MK864288
Supplier: AVANTOR PERFORMANCE MATERIAL LLC

Description: Lot analysis on label.
Catalog Number: JT4204-5
Supplier: AVANTOR PERFORMANCE MATERIAL LLC

Catalog Number: EM8.18710.5000
Supplier: MilliporeSigma

Catalog Number: EM1.08487.9025
Supplier: MilliporeSigma

Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C
Catalog Number: 76177-312
Supplier: MP Biomedicals


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