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Product Image-76482-290

Fluorescein di-b-D-galactopyranoside (FDG) ≥95%

Catalog Number: (76482-290)
Supplier: AAT BIOQUEST INC
Description: Although chromogenic assays of beta-galactosidase activity (i.e. X-Gal) are still widely used, the recent advance in fluorescence instrumentation has made fluorogenic substrates (such as FDG and MUG) be several orders of magnitude more sensitive. FDG is considered to be one of the most sensitive fluorogenic substrates available for detecting beta-galactosidase.

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Image Unavailable-103011-308

Fluorescein di-b-D-galactopyranoside (FDG)

Catalog Number: (103011-308)
Supplier: Anaspec Inc
Description: Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive fluorogenic substrates available for detecting β-galactosidase. The colorless and nonfluorescent FDG is hydrolyzed to highly fluorescent fluorescein, which exhibits excellent spectral properties (Ex/Em=492/520 nm) that match the optimal detection window of most fluorescence instruments. Galactosidase-catalyzed hydrolysis of FDG can be followed by fluorescence increase around 520 nm. Alternatively, FDG can also be used to detect β-galactosidase in a chromogenic mode since the enzymatic product (fluorescein) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). In general, fluorescence-based measurements can be several orders of magnitude more sensitive than absorption-based measurements. FDG has been widely used for identifying lacZ-positive cells with fluorescence microscopy and flow cytometry. FDG is also used to detect β-galactosidase expression in live cells. Fluorescence-based assays employing FDG are also reported to be 100 to 1000-fold more sensitive than radioisotope-based ELISAs.
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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The original product is no longer available. The replacement shown is available.
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