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You Searched For: Enzymes

Enzymes accelerate, or catalyze, chemical reactions, and they are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.

Enzymes accelerate, or catalyze, chemical reactions, and they are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.


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VWR® Pepsin

Supplier: VWR
Description: Pepsin is a proteolytic enzyme product derived from porcine stomach linings.

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J.T.Baker® Endonuclease

Supplier: AVANTOR PERFORMANCE MATERIAL LLC
Description: J.T.Baker® Endonuclease Biotech Reagent meets the strictest cGMP standards and is designed for the degradation of both single stranded and double stranded DNA and RNA. J.T.Baker® Endonuclease Biotech Reagent is used to ensure host cell DNA impurities are removed; driving process efficiency by lowering viscosity and preventing aggregation. J.T.Baker® Endonuclease Biotech Reagent is an enzyme based upon the native endonuclease of Serratia marcescens, enabling rapid clearance of residual DNA and RNA during the production and purification of both recombinant proteins and viral vectors. Non-animal origin. Absence of proteolytic activity. The purity of materials in non-negotiable. J.T.Baker® Endonuclease Biotech Reagent acts to degrade and eliminate extraneous genetic material, ensuring the pristine quality of your final product.

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VWR® Trypsin 1:250, Tissue Culture Grade

Supplier: VWR
Description: Trypsin 1:250, Tissue Culture Grade.

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Bovine DNase (from Pancreas)

Catalog Number: (97062-106)
Supplier: VWR
Description: Bovine DNase (from Pancreas)

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Bovine Deoxyribonuclease I. (from Pancreas), MP Biomedicals

Supplier: MP Biomedicals
Description: Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The average chain of limit digest is a tetranucleotide. DNase I acts upon single chain DNA, and upon double-stranded DNA and chromatin.

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Alcohol Dehydrogenase (from Yeast), MP Biomedicals

Supplier: MP Biomedicals
Description: Alcohol dehydrogenases are a group of dehydrogenase enzymes that occur in many organisms. It is a metalloenzyme containing four tightly bound zinc atoms per molecule. Each subunit also contains a second zinc atom (conformational zinc), which stabilizes the enzyme’s tertiary structure. Per subunit, there are two distinct active site sulfhydryl groups which can be distinguished on the basis of differential reactivity with iodoacetate and butyl isocyanate. A histidine residue is considered to have an essential role.

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Bovine Thrombin, MP Biomedicals

Supplier: MP Biomedicals
Description: Thrombin is prepared from fresh bovine plasma by an ion exchange method

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Pineapple Bromelain, MP Biomedicals

Supplier: MP Biomedicals
Description: Bromelain is a glycoprotein which is a highly active thiol proteinase (non-specific cysteine protease), which splits glycine esters but not lysine esters; it is also a protein-digesting and milk-clotting enzyme. Substrates susceptible to bromelain include all of the common protein materials such as gelatin, casein, gluten, collagen, elastin, globulins and muscle fiber protein.

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Alkaline Phosphatase, MP Biomedicals

Catalog Number: (IC19068001)
Supplier: MP Biomedicals
Description: Dissolves readily at 5 mg/mL in a 0.025M glychine/NaOH pH 9.6 containing 1 mM magnesium chloride, 0.1 mM zinc chloride and 10% glycerol, to give a clear pale pink solution also soluble in distilled water or dilute buffer.
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Chicken Lysozyme Type VI (from Egg White), MP Biomedicals

Catalog Number: (76177-422)
Supplier: MP Biomedicals
Description: Lysozyme (muramidase) hydrolyzes preferentially the b-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus.
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Bromelain (from Stem)

Supplier: THERMO FISHER SCIENTIFIC CHEMICALS
Description: Bromelain (from Stem)

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Salmonella Minnesota Lipid A

Catalog Number: (89152-792)
Supplier: Enzo Life Sciences
Description: Isolated from <i>Salmonella minnesota</i> R595 (Re mutant)
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Matrix Metalloproteinase

Catalog Number: (89156-414)
Supplier: Enzo Life Sciences
Description: Enzyme regulation and kinetics, comparative studies of substrate or inhibitor specificities, cleavage of target proteins. Assay or digest conditions can vary widely, but concentrations for the MMP enzymes can range between 10 and 300nM, or higher. Reaction temperatures can be between 25 and 37°C, and reaction times can range from 10 min to overnight, again depending on application and substrate. A typical assay buffer is 50mM HEPES, pH 7.0, 10mM CaCl2, 0.05% Brij-35. For more information, contact Enzo Life Sciences Technical Support.
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Exonuclease I

Catalog Number: (95040-316)
Supplier: GE Healthcare - Life Sciences
Description: Exonuclease I acts specifically on single-stranded DNA degrading it processively in the 3'- to 5'-direction, producing 5'-mononucleotides. Applications include eliminating residual single-stranded DNA containing a 3'-terminus, measuring endonucleolytic cleavage of covalently closed circular (ccc) ssDNA, measuring DNA helicase activity.
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Proteinase K

Catalog Number: (89165-712)
Supplier: Enzo Life Sciences
Description: ENZO Proteinase K is specifically designed for use in the pretreatment of formalin-fixed tissue and paraformaldehyde-fixed cells in preparation for in situ hybridization and detection studies.
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Neuraminidase, MP Biomedicals

Supplier: MP Biomedicals
Description: One unit will liberate 1.0 µmole of N-acetyl-neuraminic acid (NANA) per minute at pH 5.0 at 37°C, using either one NAN-lactose, bovine submaxillary mucin, or colominic acid as a substrate.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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