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Description: This Thermo Scientific™ formulation of Brij™ 35 is a stabilized 30% (w/v) solution of Brij-35 detergent for use in various protein methods.

Catalog Number: PI20150

Supplier: Invitrogen

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Description: The Thermo Scientific™ pMCS Tluc16-DD Vector is a multiple cloning site (MCS) vector designed to accept a promoter sequence for study of gene regulation using the intracellular TurboLuc™16 (Tluc16) luciferase reporter.Intracellular Tluc16 luciferase gene, optimized for high expression in mammalian systemsMultiple unique cloning sites provide versatility for transfer of regulatory elements from one vector to anotherDual-destabilization (DD) technology reduces accumulation of Tluc16 luciferase mRNA and protein in cells, enhancing the responsiveness of the assaySynthetic polyA terminator and Transcriptional Pause Site (TPS) included upstream of MCS to minimize non-specific transcriptional read-throughTluc16 luciferase is a 16 kDa, novel, intracellular luciferase derived from the marine copedod Metridia luciferase family

Catalog Number: PI88231

Supplier: Invitrogen

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Description: The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Streptavidin) provides mass spectrometry-friendly reagents and an optimized protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.MS-compatible—reagents directly compatible with in-solution peptide digestion, enriched samples contain minimal detergent residuals detected using LC-MSSensitive—procedure successfully enriches low abundance proteins (low ng range)Low background—binding, wash, and elution buffers optimized to minimize enrichment of background proteinsReduced antibody contamination—antibody biotinylation reduces contamination in the eluate when compared to other IP methodsRobust—procedure and reagents have been robustly tested with numerous targets to ensure consistent enrichment of low abundant proteins (ng range) with at least 2 peptides identified per proteinThe Pierce MS-Compatible Magnetic IP Kit (Streptavidin) uses high-quality Pierce Streptavidin Magnetic Beads and optimized buffers that are compatible with downstream LC-MS sample preparation and analysis

Catalog Number: PI90408

Supplier: Invitrogen

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Description: The Thermo Scientific Pierce Classic Magnetic IP/Co-IP Kit enables highly effective and efficient immunoprecipitation (IP) and co-immunoprecipitation (co-IP) of antigens and protein-complexes using less than 10 µg of antibody and a magnetic separator.

Catalog Number: PI88804

Supplier: Invitrogen

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Description: The Thermo Scientific Pierce™ classic IP kit uses high-quality Protein A/G agarose beads, microcentrifuge spin columns and buffers to perform traditional immunoprecipitation assays with ease.

Catalog Number: PI26146

Supplier: Invitrogen

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Description: Thermo Scientific EZ-Link Hydrazide-LC-Biotin is a mid-length, simple, hydrazide-activated biotinylation reagent for labeling glycoproteins and other carbohydrate-containing compounds having oxidizable sugars or aldehydes.

Catalog Number: PI21340

Supplier: Invitrogen

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Description: Thermo Scientific 50% Hydroxylamine is a 10X quenching reagent for amine-reactive isobaric mass tag labeling experiments.

Catalog Number: PI90115

Supplier: Invitrogen

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Description: Thermo Scientific Blocker Casein in PBS or TBS is a ready-to-use, solution of purified casein protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

Catalog Number: PI37528

Supplier: Invitrogen

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Description: Thermo Scientific EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Catalog Number: PI21335

Supplier: Invitrogen

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Description: Thermo Scientific Serine Hydrolase Probes are ActivX™ Fluorophosphonate (FP) and other tagged phosphonate probes to purify or assay serine hydrolase active sites using fluorescence, biotin-affinity, or mass spectrometry.

Catalog Number: PI88317

Supplier: Invitrogen

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Description: The Melon Gel Spin Plate Kit for IgG Screening offers a high-throughput format for quick purification and screening of antibodies.

Catalog Number: PI45208

Supplier: Invitrogen

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Description: The Thermo Scientific Pierce Crosslink Magnetic IP/Co-IP Kit uses crosslinking chemistry to covalently immobilize IP antibodies onto premium-quality Protein A/G Magnetic Beads for effective immunoprecipitation and co-immunoprecipitation.

Catalog Number: PI88805

Supplier: Invitrogen

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Description: These Iodination Beads are polystyrene beads that are coated with an oxidizing reagent, which provides efficient activation of ¹²⁵I for protein or peptide iodination procedures.

Catalog Number: PI28665

Supplier: Invitrogen

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Description: Pierce™ Recombinant Alkaline Phosphatase Conjugated Protein A/G is useful to probe and detect antibodies, especially IgG isotypes, of many different species hosts in Western blotting, ELISA, IHC and other immunoassay protocols.

Catalog Number: PI32391

Supplier: Invitrogen

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Description: Thermo Scientific Pierce™ Trifluoroacetic Acid (TFA), Sequencing grade is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

Catalog Number: PI85183

Supplier: Invitrogen

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Description: Pierce™ Quantitative Peroxide Assay Kit (Aqueous) detects and measures hydrogen peroxide levels in biological samples using an Iron and Xylenol orange* (XO) reagent.

Catalog Number: PI23280

Supplier: Invitrogen

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