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Description: CAS Number: 19088-73-4 MDL Number: MFCD00037480 Molecular Formula: C10H5NO3 Molecular Weight: 187.15 Purity/Analysis Method: <gt/>98.0% (GC) Form: Crystal Melting point (°C): 280 Lambda max.: 450 nm (MeOH)
Catalog Number: TCC2834-1G
Supplier: TCI America

Description: 3-Cyano-7-hydroxycoumarin is used as a calibration standard for 3-cyano-7-hydroxycoumarin-based enzyme substrates.
Catalog Number: 76480-480
Supplier: AAT BIOQUEST INC

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Description: Phenolic dyes with high pKas - such as 7-hydroxycoumarin (often called umbelliferone) and the more common 7-hydroxy-4-methylcoumarin - are not fully deprotonated and therefore not fully fluorescent unless the pH of the reaction mixture is raised to above pH ~10. Thus, substrates derived from these fluorophores are seldom used for continuous measurement of enzymatic activity in solution or live cells. The similar 3-cyano-7-hydroxycoumarin and 6,8-difluoro-7-hydroxy-4-methylcoumarin have lower pKas, making them suitable for a broader range of applications. Moreover, the -trifluoromethylumbelliferone product has longer-wavelength spectra and stronger absorbance than -methylumbelliferone. Ether, ester and phosphate substrates derived from these phenolic dyes may be fluorescent but invariably exhibit shorter-wavelength absorption and emission spectra.
Catalog Number: 102987-526
Supplier: Adipogen


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