PBST 10X

Supplier: Bandelin Electronic GmbH &Co.KG
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Avg. Ratings: out of 5

MB-075-1000
RLMB-075-1000EA 130.6 USD
RLMB-075-1000
PBST 10X
Buffers Electrophoresis Buffers Washing Buffers

Phosphate Buffer Saline-Tween (PBST) 10X


  • 10X concentrated stock solution
  • 0.2 M Potassium Phosphate, 1.5 M Sodium Chloride and 0.5% (v/v) Tween-20 at a pH of 7.2
  • Aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers
  • Tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria
  • Use in assay washing procedure


Phosphate Buffer Saline-Tween is commonly use in washing procedure while performing western-blot, ELISA and, more generally immuno-assays.


Application Note: This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration.  Dilute only prior to immediate use. 10X Phosphate Buffered Saline (PBS) consists of 0.2 M Potassium Phosphate, 1.5 M Sodium Chloride and 0.5% (v/v) Tween-20 at a pH of 7.2. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.

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